Mechanism of long non-coding RNA MAGI2- antisense RNA 3 targeting microRNA-31-5p to regulate proliferation and metastasis of SiHa cells in cervical cancer
By: Yang, Jingyan
.
Contributor(s): Chen, Jing.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(2), Mar-Apr.Description: 477-482p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To investigate the effect of long non coding RNA MAGI2-antisense RNA 3 on the proliferation, invasion
and migration of cervical cancer cells and the underlying mechanism. SiHa cells were divided into plasmid
cloning DNA group, plasmid cloning DNA-MAGI2-antisense RNA 3 group, anti-microRNA-negative
control group, anti-microRNA-31-5p group, plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA-
negative control group and plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA-31-5p group;
quantitative reverse transcription polymerase chain reaction was used to determine the messenger
RNA expression levels of microRNA-31-5p and MAGI2-antisense RNA 3; cell viability was detected by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay; the number of cell migration and
invasion in each group was detected by Transwell assay; dual luciferase reporter assay was performed to
detect the fluorescence activity. The expression levels of microRNA-31-5p were significantly increased and
those of MAGI2-antisense RNA 3 messenger RNA were decreased in cervical cancer cell lines (p<0.05);
overexpression of MAGI2-antisense RNA 3 or inhibition expression of microRNA-31-5p could suppress
SiHa cell proliferation, migration and invasion (p<0.05). MAGI2-antisense RNA 3 targeted regulation
of microRNA-31-5p; overexpression of microRNA-31-5p partially reversed the inhibitory effects of
overexpressing MAGI2-antisense RNA 3 on the proliferation, migration, invasion of SiHa cells. Long non
coding RNA MAGI2-antisense RNA 3 can inhibit cervical cancer cell proliferation, migration and invasion
by regulating microRNA-31-5p.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2189 |
To investigate the effect of long non coding RNA MAGI2-antisense RNA 3 on the proliferation, invasion
and migration of cervical cancer cells and the underlying mechanism. SiHa cells were divided into plasmid
cloning DNA group, plasmid cloning DNA-MAGI2-antisense RNA 3 group, anti-microRNA-negative
control group, anti-microRNA-31-5p group, plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA-
negative control group and plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA-31-5p group;
quantitative reverse transcription polymerase chain reaction was used to determine the messenger
RNA expression levels of microRNA-31-5p and MAGI2-antisense RNA 3; cell viability was detected by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay; the number of cell migration and
invasion in each group was detected by Transwell assay; dual luciferase reporter assay was performed to
detect the fluorescence activity. The expression levels of microRNA-31-5p were significantly increased and
those of MAGI2-antisense RNA 3 messenger RNA were decreased in cervical cancer cell lines (p<0.05);
overexpression of MAGI2-antisense RNA 3 or inhibition expression of microRNA-31-5p could suppress
SiHa cell proliferation, migration and invasion (p<0.05). MAGI2-antisense RNA 3 targeted regulation
of microRNA-31-5p; overexpression of microRNA-31-5p partially reversed the inhibitory effects of
overexpressing MAGI2-antisense RNA 3 on the proliferation, migration, invasion of SiHa cells. Long non
coding RNA MAGI2-antisense RNA 3 can inhibit cervical cancer cell proliferation, migration and invasion
by regulating microRNA-31-5p.
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