Su, Jing
Molecular mechanism of circular rna molecule circsetd3 in gefitinib acquired resistance in non-small cell lung cancer - Vol.84(5), Sep-Oct - Mumbai Indian Journal of Pharmaceutical Science 2022 - 1279-1287p.
To explore the molecular pathway of the circular RNA molecule in gefitinib acquired resistance in non-
small cell lung cancer and to provide a new therapeutic direction for clinical treatment of non-small cell
lung cancer. The gefitinib-sensitive non-small cell lung cancer cell strain H292_S was selected to construct
gefitinib acquired resistant cell model H292_R. Also, the overexpression group and vector control group
of H292_S transfected circSETD3; the knockdown plasmid group and vector control group of H292_R
transfected circSETD3 were prepared. The degree of cell response to gefitinib was measured by Western
blotting and cell counting kit-8 method. The RNA sequencing method was used to screen differentially
expressed circular RNA in cells and real-time fluorescent quantitative polymerase chain reaction was used
to verify the sequencing results; the dual-luciferase report was used to screen and verify the relationship
between circSETD3 and microRNA-520h. After the drug-resistant H292_R cell strain was processed by
gefitinib, the expression of phosphorylated epidermal growth factor receptor, phosphatidylinositol 3-kinase
and protein kinase B decreased significantly. The expressions of three circular RNAs in has_circ_0000567,
has_circ_0000655 and has_circ_0006867, were all up-regulated in drug-resistant strains, and the
differences were statistically significant (p<0.05). The overexpression group of circSETD3 had a significant
effect on the growth and reproduction ability of cells (p<0.05); the wild type group of the dual-luciferase
report plasmid was transfected into 293T cells together with microRNA-520h mimics and the fluorescence
intensity decreased significantly (p<0.05). In addition, if the luciferase reporter plasmid mutant group
(mutant) was transfected into 293T cells together with microRNA-520h mimics, the fluorescence intensity
would not change significantly. circSETD3 could be directly bound to microRNA-520h.
PHARMACEUTICS
Molecular mechanism of circular rna molecule circsetd3 in gefitinib acquired resistance in non-small cell lung cancer - Vol.84(5), Sep-Oct - Mumbai Indian Journal of Pharmaceutical Science 2022 - 1279-1287p.
To explore the molecular pathway of the circular RNA molecule in gefitinib acquired resistance in non-
small cell lung cancer and to provide a new therapeutic direction for clinical treatment of non-small cell
lung cancer. The gefitinib-sensitive non-small cell lung cancer cell strain H292_S was selected to construct
gefitinib acquired resistant cell model H292_R. Also, the overexpression group and vector control group
of H292_S transfected circSETD3; the knockdown plasmid group and vector control group of H292_R
transfected circSETD3 were prepared. The degree of cell response to gefitinib was measured by Western
blotting and cell counting kit-8 method. The RNA sequencing method was used to screen differentially
expressed circular RNA in cells and real-time fluorescent quantitative polymerase chain reaction was used
to verify the sequencing results; the dual-luciferase report was used to screen and verify the relationship
between circSETD3 and microRNA-520h. After the drug-resistant H292_R cell strain was processed by
gefitinib, the expression of phosphorylated epidermal growth factor receptor, phosphatidylinositol 3-kinase
and protein kinase B decreased significantly. The expressions of three circular RNAs in has_circ_0000567,
has_circ_0000655 and has_circ_0006867, were all up-regulated in drug-resistant strains, and the
differences were statistically significant (p<0.05). The overexpression group of circSETD3 had a significant
effect on the growth and reproduction ability of cells (p<0.05); the wild type group of the dual-luciferase
report plasmid was transfected into 293T cells together with microRNA-520h mimics and the fluorescence
intensity decreased significantly (p<0.05). In addition, if the luciferase reporter plasmid mutant group
(mutant) was transfected into 293T cells together with microRNA-520h mimics, the fluorescence intensity
would not change significantly. circSETD3 could be directly bound to microRNA-520h.
PHARMACEUTICS