Normal view MARC view ISBD view

Molecular mechanism of circular rna molecule circsetd3 in gefitinib acquired resistance in non-small cell lung cancer

By: Su, Jing.
Contributor(s): Zhao, J.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(5), Sep-Oct.Description: 1279-1287p.Subject(s): PHARMACEUTICSOnline resources: Click here In: Indian journal of pharmaceutical sciencesSummary: To explore the molecular pathway of the circular RNA molecule in gefitinib acquired resistance in non- small cell lung cancer and to provide a new therapeutic direction for clinical treatment of non-small cell lung cancer. The gefitinib-sensitive non-small cell lung cancer cell strain H292_S was selected to construct gefitinib acquired resistant cell model H292_R. Also, the overexpression group and vector control group of H292_S transfected circSETD3; the knockdown plasmid group and vector control group of H292_R transfected circSETD3 were prepared. The degree of cell response to gefitinib was measured by Western blotting and cell counting kit-8 method. The RNA sequencing method was used to screen differentially expressed circular RNA in cells and real-time fluorescent quantitative polymerase chain reaction was used to verify the sequencing results; the dual-luciferase report was used to screen and verify the relationship between circSETD3 and microRNA-520h. After the drug-resistant H292_R cell strain was processed by gefitinib, the expression of phosphorylated epidermal growth factor receptor, phosphatidylinositol 3-kinase and protein kinase B decreased significantly. The expressions of three circular RNAs in has_circ_0000567, has_circ_0000655 and has_circ_0006867, were all up-regulated in drug-resistant strains, and the differences were statistically significant (p<0.05). The overexpression group of circSETD3 had a significant effect on the growth and reproduction ability of cells (p<0.05); the wild type group of the dual-luciferase report plasmid was transfected into 293T cells together with microRNA-520h mimics and the fluorescence intensity decreased significantly (p<0.05). In addition, if the luciferase reporter plasmid mutant group (mutant) was transfected into 293T cells together with microRNA-520h mimics, the fluorescence intensity would not change significantly. circSETD3 could be directly bound to microRNA-520h.
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Call number Status Date due Barcode Item holds
Articles Abstract Database Articles Abstract Database School of Pharmacy
Archieval Section
Not for loan 2023-1140
Total holds: 0

To explore the molecular pathway of the circular RNA molecule in gefitinib acquired resistance in non-
small cell lung cancer and to provide a new therapeutic direction for clinical treatment of non-small cell
lung cancer. The gefitinib-sensitive non-small cell lung cancer cell strain H292_S was selected to construct
gefitinib acquired resistant cell model H292_R. Also, the overexpression group and vector control group
of H292_S transfected circSETD3; the knockdown plasmid group and vector control group of H292_R
transfected circSETD3 were prepared. The degree of cell response to gefitinib was measured by Western
blotting and cell counting kit-8 method. The RNA sequencing method was used to screen differentially
expressed circular RNA in cells and real-time fluorescent quantitative polymerase chain reaction was used
to verify the sequencing results; the dual-luciferase report was used to screen and verify the relationship
between circSETD3 and microRNA-520h. After the drug-resistant H292_R cell strain was processed by
gefitinib, the expression of phosphorylated epidermal growth factor receptor, phosphatidylinositol 3-kinase
and protein kinase B decreased significantly. The expressions of three circular RNAs in has_circ_0000567,
has_circ_0000655 and has_circ_0006867, were all up-regulated in drug-resistant strains, and the
differences were statistically significant (p<0.05). The overexpression group of circSETD3 had a significant
effect on the growth and reproduction ability of cells (p<0.05); the wild type group of the dual-luciferase
report plasmid was transfected into 293T cells together with microRNA-520h mimics and the fluorescence
intensity decreased significantly (p<0.05). In addition, if the luciferase reporter plasmid mutant group
(mutant) was transfected into 293T cells together with microRNA-520h mimics, the fluorescence intensity
would not change significantly. circSETD3 could be directly bound to microRNA-520h.

There are no comments for this item.

Log in to your account to post a comment.

Click on an image to view it in the image viewer

Unique Visitors hit counter Total Page Views free counter
Implemented and Maintained by AIKTC-KRRC (Central Library).
For any Suggestions/Query Contact to library or Email: librarian@aiktc.ac.in | Ph:+91 22 27481247
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.

Powered by Koha