Molecular mechanism of circular rna molecule circsetd3 in gefitinib acquired resistance in non-small cell lung cancer (Record no. 19729)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20230811101521.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 230811b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 21501 |
| Author | Su, Jing |
| 245 ## - TITLE STATEMENT | |
| Title | Molecular mechanism of circular rna molecule circsetd3 in gefitinib acquired resistance in non-small cell lung cancer |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.84(5), Sep-Oct |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mumbai |
| Name of publisher, distributor, etc. | Indian Journal of Pharmaceutical Science |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 1279-1287p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | To explore the molecular pathway of the circular RNA molecule in gefitinib acquired resistance in non- small cell lung cancer and to provide a new therapeutic direction for clinical treatment of non-small cell lung cancer. The gefitinib-sensitive non-small cell lung cancer cell strain H292_S was selected to construct gefitinib acquired resistant cell model H292_R. Also, the overexpression group and vector control group of H292_S transfected circSETD3; the knockdown plasmid group and vector control group of H292_R transfected circSETD3 were prepared. The degree of cell response to gefitinib was measured by Western blotting and cell counting kit-8 method. The RNA sequencing method was used to screen differentially expressed circular RNA in cells and real-time fluorescent quantitative polymerase chain reaction was used to verify the sequencing results; the dual-luciferase report was used to screen and verify the relationship between circSETD3 and microRNA-520h. After the drug-resistant H292_R cell strain was processed by gefitinib, the expression of phosphorylated epidermal growth factor receptor, phosphatidylinositol 3-kinase and protein kinase B decreased significantly. The expressions of three circular RNAs in has_circ_0000567, has_circ_0000655 and has_circ_0006867, were all up-regulated in drug-resistant strains, and the differences were statistically significant (p<0.05). The overexpression group of circSETD3 had a significant effect on the growth and reproduction ability of cells (p<0.05); the wild type group of the dual-luciferase report plasmid was transfected into 293T cells together with microRNA-520h mimics and the fluorescence intensity decreased significantly (p<0.05). In addition, if the luciferase reporter plasmid mutant group (mutant) was transfected into 293T cells together with microRNA-520h mimics, the fluorescence intensity would not change significantly. circSETD3 could be directly bound to microRNA-520h. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 21502 |
| Co-Author | Zhao, J. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Indian journal of pharmaceutical sciences |
| Place, publisher, and date of publication | New Delhi Indian Pharmaceutical Association |
| International Standard Serial Number | 0250-474X |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijpsonline.com/articles/the-molecular-mechanism-of-circular-rna-molecule-circsetd3-in-gefitinib-acquired-resistance-in-nonsmall-cell-lung-cancer.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| School of Pharmacy | School of Pharmacy | Archieval Section | 2023-08-11 | 2023-1140 | 2023-08-11 | 2023-08-11 | Articles Abstract Database |